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主营:分子类,蛋白类,抗体类,生化类试剂
℡ 4000-520-616
℡ 4000-520-616
asuragen/AmplideX® PCR/CE FMR1 Reagents/24 UL/49513
产品编号:49513
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场      地:美国(厂家直采)
联系QQ:1570468124
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品      牌: asuragen
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asuragen/AmplideX® PCR/CE FMR1 Reagents/24 UL/49513
商品介绍

AmplideX® PCR/CE FMR1 Reagents

AmplideX FMR1AmplideX PCR/CE FMR1 Reagents* are market-leading research tools for the detection of CGG repeats in the fragile X mental retardation (FMR1) gene. These reagents provide a PCR-only approach based on Triplet Repeat Primed PCR (TP-PCR) design to reliably amplify and detect all alleles including Full Mutations.

Features & Benefits

AmplideX PCR/CE FMR1 Reagents* have created an easy-to-use, accessible, high performance method for laboratories to reliably analyze CGG repeats and detect interrupting AGG sequences in the FMR1 gene.

Reduced ComplexityEase-of-analysis of the FMR1 gene has been simplified through:

  • Implementation of proprietary PCR solution for amplifying GC-rich regions
  • Automation of result calling using AmplideX PCR/CE FMR1 Reporter* 

Optimized WorkflowValuable operator hands-on time has been significantly reduced through:

  • Direct injection of PCR products (no PCR clean up) in to Capillary Electrophoresis platforms
  • Decreased need for Southern blot analysis (up to 50 fold)
  • End-to-end solution for FMR1 analysis including all necessary reagents and software

Quality PerformancePerforming FMR1 Analysis with Greater Sensitivity and Accuracy:

  • Detection of all allele expansions, including low abundance full mutation size mosaics with up to at least 1300 CGG repeats
  • Up to 875 fold more sensitive than Southern blot1
  • Resolution of female homozygous and heterozygous samples and indication of interrupting AGG sequences
  • Proven performance as indicated by more than 30 peer reviewed publications

*For Research Use Only. Not for use in diagnostic procedures.

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Product Description

Analytical Characteristics of AmplideX PCR/CE FMR1 Reagents*:

  • Detects all alleles including low abundance full mutations (Figure 1)
  • Accurately sizes any repeat up to 200 CGG repeats (Figure 2)
  • Resolves female zygosity (Figure 3)
  • Detects presence of AGG interruptions (Figure 4)

Figure 1: Amplification of Asuragen’s Methylation and Sensitivity Control which has a 5% full mutation in a background of 95% Normal

AmplideX Methylation and Sensitivity Control

Figure 2. Female premutation sample with accurate sizing of Normal (30 CGG) and Pre mutation allele (56 CGG)

AmplideX Female premutation sample

Figure 3: The difference in the “stutter” peak patterns of homozygous and heterozygous female provides a clear resolution of zygosity

AmplideX resolves female zygosity

Figure 4. Female Full Mutation sample with AGG interruptions as indicated by sudden decrease in peak heights of the “stutter” peak profile

AmplideX Female Full Mutation sample

Ordering

Product NameNumber of ReactionsCatalog Number
AmplideX PCR/CE FMR1 Control24 UL49513
AmplideX mPCR FMR1 Control24 UL49514
AmplideX PCR/CE FMR1 Reagents10049402
AmplideX mPCR FMR1 Kit2449442
AmplideX PCR/CE FMR1 ReporterN/A49576

T 1-877-777-1874; 512-681-5200 F 512-681-5202 E orders@asuragen.com

View Sales Contacts

References

  1. Referenced in over 30 peer reviewed publications and used in over 200 laboratories, the AmplideX® PCR/CE FMR1 Reagents* are globally recognized as best-in-class for assessment of CGG repeats in the FMR1 gene.
    • Key resources
    • Videos

AmplideX® PCR/CE FMR1 Kit

AmplideX FMR1AmplideX PCR/CE FMR1 Kit is an in vitro diagnostic (IVD) device for use in clinical laboratories for detection of the CGG repeats in the fragile X mental retardation (FMR1) gene. The device is intended to aid in the diagnosis of fragile X syndrome and fragile X associated disorders, e.g. tremor and ataxia syndrome (FX-TAS) and primary ovarian insufficiency (FXPOI), through determination of CGG repeat length up to 200 CGG and detection of alleles greater than 200 CGG. The kit provides a PCR-only approach based on Triplet Repeat Primed PCR (TP-PCR) design to reliably amplify and detect all alleles including Full Mutations.

Features & Benefits

AmplideX PCR/CE FMR1 Kit has created an easy-to-use, accessible, high performance method for laboratories to reliably analyze CGG repeats and detect interrupting AGG sequences in the FMR1 gene.

Reduced ComplexityEase-of-analysis of the FMR1 gene has been simplified through:

  • Implementation of proprietary PCR solution for amplifying GC-rich regions
  • Automation of result calling using AmplideX PCR/CE FMR1 Reporter 

Optimized WorkflowValuable operator hands-on time has been significantly reduced through:

  • Direct injection of PCR products (no PCR clean up) in to Capillary Electrophoresis platforms
  • Decreased need for Southern blot analysis (up to 50 fold)
  • End-to-end solution for FMR1 analysis including all necessary reagents and software

Quality PerformancePerforming FMR1 Analysis with Greater Sensitivity and Accuracy:

  • Detection of all allele expansions, including low abundance full mutation size mosaics with up to at least 1300 CGG repeats
  • Up to 875 fold more sensitive than Southern blot1
  • Resolution of female homozygous and heterozygous samples and indication of interrupting AGG sequences
  • Proven performance as indicated by more than 30 peer reviewed publications

Product Description

Analytical Characteristics of AmplideX PCR/CE FMR1 Kit:

  • Proven clinical accuracy compared to Southern Blot (Table 1)
  • Detects all alleles including low abundance full mutations (Figure 1)
  • Accurately sizes all alleles up to 200 CGG repeats (Figure 2)
  • Resolves female zygosity (Figure 3)
  • Detects presence of AGG interruptions (Figure 4)

Table 1: Diagnostic Sensitivity of 100%; Diagnostic Specificity of 98.4% and Overall Accuracy of 99%AmplideX has proven clinical accuracy compared to Southern Blot*These 2 samples presented premutation alleles by both methods and low intensity full mutation alleles detected only by the AmplideX PCR/CE FMR1 Kit

Figure 1: Amplification of Asuragen’s Methylation and Sensitivity Control which has a 5% full mutation in a background of 95% Normal

AmplideX Methylation and Sensitivity Control

Figure 2. Female Pre-mutation sample with accurate sizing of Normal (30 CGG) and Pre-mutation allele (56 CGG)

AmplideX Female premutation sample

Figure 3: The difference in the “stutter” peak patterns of homozygous and heterozygous female provides a clear resolution of zygosity

AmplideX resolves female zygosity

Figure 4. Female Full Mutation sample with AGG interruptions as indicated by sudden decrease in peak heights of the “stutter” peak profile

AmplideX Female Full Mutation sample

Ordering

Product NameNumber of ReactionsCatalog Number
AmplideX PCR/CE FMR1 Control*24 UL49513
AmplideX mPCR FMR1 Control*24 UL49514
AmplideX PCR/CE FMR1 Reagents*10049402
AmplideX PCR/CE FMR1 Kit10076008
AmplideX mPCR FMR1 Kit*2449442
AmplideX PCR/CE FMR1 Reporter*N/A49576

T 1-877-777-1874; 512-681-5200 F 1-512-681-5202 E orders@asuragen.com

View Sales Contacts

References

  1. Referenced in over 30 peer reviewed publications and used in over 200 laboratories, the AmplideX® PCR/CE FMR1 Reagents* are globally recognized as best-in-class for assessment of CGG repeats in the FMR1 gene.
    • Key resources
    • Videos
品牌介绍

asuragen的AmplideX®PCR / CE C9orf72试剂盒

C9orf72主网页图像小AmplideX PCR / CE C9orf72试剂盒(RUO)是用于检测C9orf72基因中GGGGCC重复序列的研究产品。这些试剂提供了基于重复引物PCR(RP-PCR)设计的单管PCR方法,以分析C9orf72基因中的重复序列  。


 


特点与优势

分析特性

订购方式

特点与优势

Asuragen已经建立了市场领先的技术,可以可靠地扩增和分析FMR1基因中CGG三联体重复序列。AmplideX PCR / CE C9orf72 试剂盒(RUO)是该技术向神经遗传学领域的扩展。该试剂盒为临床和药学研究人员提供了一种可靠且可重现的方法,可用于C9orf72基因中六核苷酸重复扩增的高分辨率基因分型  ,这对额颞叶痴呆(FTD)引起了越来越多的关注。疾病-肌萎缩性侧索硬化症(ALS)。



通过以下方法简化了 C9orf72基因的复杂度分析:


实施专有的二合一PCR解决方案以扩增富含GC的区域

单一来源的试剂盒,包含C9orf72  重复扩增所需的所有PCR试剂

简化的工作流程,动手时间最短

优化的工作流程

通过以下方式大大减少了宝贵的操作员动手时间:


单一PCR反应可用于上浆和筛选

直接将PCR产物注入(无需PCR清理)到毛细管电泳平台

减少对Southern blot分析的需求

质量绩效

执行C9orf72一个具有更高的灵敏度和准确度nalysis:


等位基因大小的长度提高了五倍:精确定量多达145个重复序列

检测到大于145个重复的等位基因

揭示低水平的镶嵌和次要等位基因


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